Groups Involved

Capacities of each host institution in terms of research expertise.

Nb Partner name Research expertise
1.
Alain
MILON,
Pascal
DEMANGE
-
Membrane protein biophysics, lipid – protein interactions
-
NMR liquid and solid state (2H, 13C, 15N, 31P, 1D-3D), lipid dynamics, protein structure, oriented bilayers, triple resonance MAS, ligand screening (DOSY, trNOE, STD)
-
Expression – purification of GPCRs in the methylotrophic yeast P. pastoris and in E. coli
-
Stable isotope labelling
2.
Jean Luc
POPOT
-
Membrane protein biochemistry: expression, overexpression under native form or as inclusion bodies, cell-free synthesis, solubilization, stabilization, purification, crystallization, renaturation, SEC and AUC analyses, immobilization, ligand binding...
-
Membrane protein biophysics: X ray crystallography, solution and solid-state NMR, SANS, SAXS, AUC, spectroscopy (UV-visible, fluorescence, FRET, CD and SRCD), etc.
-
Development of novel surfactants (amphipols, fluorinated surfactants): chem.., phys. Chem.., development of applications in membrane protein biochem. and biophys.
3.
Eva
PEBAYPEYROULA
-
Membrane protein crystallization and crystallography
-
Expression, purification and refolding of GPCRs in E. coli
-
Biophysical studies in solution
4.
Valentin
GORDELIY
-
Membrane protein crystallization
-
Lipidic phases : Biophysical characterization and use for crystallization of membrane protein complexes
5.
Dr MIKOL
Vincent
-
Structural biology of protein of pharmacological relevance (X ray crystallography, NMR, SMS, …).
-
Drug Design
-
Protein ligand interaction.
6.
Slawomir
FILIPEK
-
Homology modelling
-
Molecular dynamics in membranes
-
Steered molecular dynamics for protein unfolding and domain movement
-
Construction of complexes of membrane proteins
-
Activation and inactivation processes of GPCRs
-
Ligand docking, structure based drug design
7.
Margarida
ARCHER
-
X-ray Crystallography (small and large molecules)
-
Biochemical characterization
-
Structure-function relationship of enzymes/protein complexes involved in metabolism and respiration
-
Expression and purification of membrane transporters in E. coli 
8.
Horst
VOGEL
-
Heterologous expression of various GPCRs including odorant receptors
-
Investigating cellular signalling reactions in live cells using selective fluorescence labelling and (single molecule) microscopic imaging
-
Purification of GPCRs
-
Observation of activation of GPCRs and subsequent downstream processes by SPR and fluorescence spectroscopies using reconstitued
9.
Andreas
ENGEL
-
Heterologous expression of various GPCRs including odorant receptors
-
protein purification and 2D crystallization
-
Electron crystallography & image processing
-
high-resolution AFM
10.
Daniel
MÜLLER
-
High-resolution AFM imaging of the structure and assembly of native membrane proteins
-
AFM imaging of native membrane proteins at work
-
SMFS to detect molecular interactions of membrane proteins
-
Dynamic force spectroscopy to characterize energy landscape of membrane proteins
-
Development of new AFM and SMFS techniques to characterize cell membranes and membrane proteins
11.
Hartmut
OSCHKINAT
-
Solid-state NMR structure determination of proteins
-
Development of a methodology for membrane protein structure determination by solid-state NMR
-
Development of new MAS NMR techniques
12.
Volker
DOETSCH,
Frank
BERNHARD
-
Liquid state NMR based structure determination of soluble proteins and of membrane proteins
-
Cell free expression of procaryotic and of eucaryotic membrane proteins
-
Functional characterization of membrane proteins
13.
Frank
PELZER
-
Development of nano-analytic instrumentsparticularly atomie force microscope (AFM) systems and for research in life sciences/soft matter applications; high resolution imaging, force measurement, nanomanipulation and nanolithography
14.
Marc
BALDUS
-
Solid-state NMR methods for the determination of structure and dynamics of molecular complexes, including membrane-embedded proteins and ligand-receptor complexes.
-
Bacterial expression, purification and functional reconstitution of membrane proteins in lipid bilayers.
15.
R. Helmut
GRUBMÜLL
-
The aim of the theoretical biophysics group is to contribute to the understanding of the physics and function of biomolecules - particularly proteins - at the atomic level
-
Development of the parallel molecular dynamics program EGO
16.
Gregg
SIEGAL
-
Small molecule-protein interactions, high throughput, NMR-based ligand screening, protein structure determination, solution and imaging NMR methods, protein immobilization